FastQCFastQC Report
Sat 23 Jun 2018
HJV33BCX2_l01_n02_ATAC_Root_Rep2_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV33BCX2_l01_n02_ATAC_Root_Rep2_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30932508
Sequences flagged as poor quality0
Sequence length51
%GC63

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGACGC895550.024.99426545
TGACGCT372300.024.8989245
TCTGACG1272900.018.24421145
ATCTGAC1578950.014.70446644
CATCTGA1773150.013.09268343
ACATCTG1983850.011.73588642
GTACTAT9650.011.4217731
GTGTAAG15350.011.2835861
CATCTTA15850.011.21658443
GTCTAGA7650.011.173441
CTAGACT6850.011.1648924
TCTTACG16600.011.11699845
GTGTAGG45050.010.9848131
CACATCT2148250.010.89419641
TATACTG10800.010.8304655
GTGTAGA44500.010.7667441
GTCTAAG7800.010.6701841
GTCCTAT15050.010.611741
TACACCG85650.010.6101525
GTACAAG55150.010.5637711
Produced by FastQC (version 0.11.5)