FastQCFastQC Report
Sat 23 Jun 2018
HJV33BCX2_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV33BCX2_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6660584
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4835057.259198292522097No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTCAC301.2820814E-436.761727
TAGTACA250.00244574434.9253354
GTTCTAT402.1247031E-532.2648741
CTTAAAT300.0055409629.5531149
ATTTACC300.005971076429.1044464
CCCTTTA406.735079E-427.7060439
ACAGTCA406.776886E-427.6767338
TCAGAGG406.776886E-427.6767338
AACTTAG406.929798E-427.5712877
CTCCGAG7200.027.33725245
ACCGATC4900.026.98972334
GCCCTAG408.0252835E-426.8873961
CTCGTAT5100.025.55144140
AGGAATG5300.025.13971522
TCTCGTA5250.024.8191939
CTTAGAG551.5159298E-424.1798239
TGATACG651.9283834E-523.5074354
GGAATGC5800.023.38092623
AGACCGA5650.022.97304232
CAGAACA1053.3669494E-922.8677774
Produced by FastQC (version 0.11.5)