FastQCFastQC Report
Sat 23 Jun 2018
HJV33BCX2_l01_n01_ATAC_Root_Rep2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV33BCX2_l01_n01_ATAC_Root_Rep2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24497891
Sequences flagged as poor quality0
Sequence length51
%GC63

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAG466150.027.27406545
TCCGAGC194050.026.3185845
TCTCCGA776950.018.01429745
ATCTCCG973500.014.4100344
CTAGACT5800.013.5844234
GTGTAAG12050.013.457021
GTCTTAG7200.013.450531
TTAGGAC7400.012.7767013
GTGTTAG9200.012.7297221
CATCTCC1118250.012.61114343
GTCTAAG6700.012.4374221
GTACTAG6350.012.0589251
ACATCTC1177800.011.94535442
GTGTAGA34200.011.7218811
GTGTTAC13450.011.7213921
GTCCTAA7850.011.4760851
TAGGACT9850.011.4270714
TACACCG69350.011.296245
GTGTAGG38350.010.9232281
CACATCT1288550.010.9046941
Produced by FastQC (version 0.11.5)