FastQCFastQC Report
Tue 12 Jun 2018
HJKMGBCX2_l02_n02_Neset10x_adult1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJKMGBCX2_l02_n02_Neset10x_adult1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177496306
Sequences flagged as poor quality0
Sequence length99
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCA7993530.45034909064530054No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG6334630.3568879906717608No Hit
GTGCCAACCCCCCTGGCCCCCCCAAAAGGGGGGTGGTGCTGGTGCAATTG3922220.22097473960951053No Hit
GGTGCATATGGTGCCAACCCCCCTGGCCCCCCCAAAAGGGGGGTGGTGCT2427120.13674200070394704No Hit
GCTAAATAAACAAGAAAGCAAAATCAACAACCGCAAAAGTCTAAGACATG2137330.12041546374491872No Hit
GGTGCCAACCCCCCTGGCCCCCCCAAAAGGGGGGTGGTGCTGGTGCAATT2053180.1156745200094474No Hit
GCCAACCCCCCTGGCCCCCCCAAAAGGGGGGTGGTGCTGGTGCAATTGCA2043330.11511957888295433No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCAGC1791930.10095590383723255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT7860900.075.948531
AGCAGTG8124250.073.304872
CAGTGGT9067450.065.601354
GCAGTGG9381400.063.4944763
AGTGGTA9365000.063.4915965
GTGGTAT9897750.060.114346
GGTATCA10070250.059.038838
TGGTATC10092900.058.9459157
GTATCAA12237450.048.8182649
CATGGGG6270900.032.908424-25
AGAGTAC10013900.032.78788418-19
GCAGAGT10103400.032.51889416-17
TACATGG10025350.032.35568622-23
CAACGCA10434100.031.76087212-13
ACGCAGA10432750.031.73116914-15
ATCAACG10556300.031.50926410-11
AGTACAT10759750.030.36513720-21
CATGGGA3354050.029.24470524-25
CAGAGTA10123450.029.1381518-19
CGCAGAG10182900.029.11034816-17
Produced by FastQC (version 0.11.5)