FastQCFastQC Report
Tue 12 Jun 2018
HJKMGBCX2_l02_n01_Neset10x_adult1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJKMGBCX2_l02_n01_Neset10x_adult1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177496306
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCGAC307650.018.478118
CAATGGA566400.018.4571598
GGAAAGC453550.018.2428231
CTGCGGA422150.018.0585561
GATGAAA519750.018.0304971
ATAACGC520300.017.916491
GACTGCG518400.017.8341481
GCGCGAT410600.017.7857821
TTGAACG473750.017.7733651
GGGAATG456250.017.6902471
CATGGCG555350.017.6449741
TGAGCCG377750.017.6296141
GGGCATC583950.017.612351
ACTGAAC441150.017.6048161
ACTGATG451000.017.5465951
TGACAAC488750.017.5332641
TGACGGC376000.017.515991
TGGCCAG523750.017.4272121
GACCTGG513200.017.4045261
TGGCTGG572750.017.3860281
Produced by FastQC (version 0.11.5)