FastQCFastQC Report
Tue 12 Jun 2018
HJKMGBCX2_l01_n02_tenx1-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJKMGBCX2_l01_n02_tenx1-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203968309
Sequences flagged as poor quality0
Sequence length99
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCA10298460.5049049065754622No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG8608130.42203271881809834No Hit
GTGCCAACCCCCCTGGCCCCCCCAAAAGGGGGGTGGTGCTGGTGCAATTG6152520.30164097698138No Hit
GGTGCATATGGTGCCAACCCCCCTGGCCCCCCCAAAAGGGGGGTGGTGCT3302470.1619109368602943No Hit
GGTGCCAACCCCCCTGGCCCCCCCAAAAGGGGGGTGGTGCTGGTGCAATT2881900.14129155720950748No Hit
GCTAAATAAACAAGAAAGCAAAATCAACAACCGCAAAAGTCTAAGACATG2788210.13669819658111693No Hit
GGTGCAATTGCAAAGGCACAGGAGCACAGGAGCAGCTAAATAAACAAGAA2639500.1294073580812988No Hit
GCAAAATCAACAACCGCAAAAGTCTAAGACATGTATCACGAATTGAAAAT2166570.10622091297526029No Hit
GCAAAAGCCAAGGCCGGAGCAGGTGCATATGGTGCCAACCCCCCTGGCCC2132050.10452849319842134No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT6617150.078.333114
AGTGGTA6646200.078.0315
GTGGTAT6885150.075.346266
GGTATCA7071050.073.3929448
AGCAGTG7106550.073.134172
TGGTATC7107750.073.099557
AAGCAGT7349700.071.136891
GCAGTGG7339550.070.764743
GTATCAA9580600.054.4913259
CATGGGG4955000.039.80037324-25
AGAGTAC7041050.038.8221318-19
GCAGAGT7081100.038.6316216-17
TACATGG7143350.037.7760622-23
ACGCAGA7434750.036.9963114-15
CAACGCA7454600.036.9937712-13
ATCAACG7614150.036.35869210-11
CGCAGAG7182250.036.18263216-17
CAGAGTA7170100.036.119218-19
ACATGGG7075800.036.0455624-25
ATGGGGG3383150.035.9270426-27
Produced by FastQC (version 0.11.5)