FastQCFastQC Report
Tue 12 Jun 2018
HJKMGBCX2_l01_n01_tenx1-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJKMGBCX2_l01_n01_tenx1-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203968309
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCGGA534750.018.5918521
CAATGGA543900.018.0659838
GGGACCT612950.018.0184971
GATGAAA561100.017.7956221
TGCTACC713650.017.7681261
GACTAAC504550.017.7239911
TGACGGC448950.017.6625671
TGGACGC576350.017.653291
ACTGAAC472650.017.4690931
CGTGAGC650350.017.410941
GGAAAGC551850.017.3581051
GCGCGAT431000.017.3120441
TTGAACG453900.017.2752721
AGGCGAC227550.017.2368668
GCATGCG536250.017.1607861
CTGAAAC536900.017.1556851
CTCGAAA475300.017.1547991
GGGAATG459000.017.101661
GATCTAG671400.017.0969521
ACGCCGA566800.017.0505961
Produced by FastQC (version 0.11.5)