FastQCFastQC Report
Tue 12 Jun 2018
HJJNMBCX2_l02_n02_Neset10x_adult2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJNMBCX2_l02_n02_Neset10x_adult2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192016477
Sequences flagged as poor quality0
Sequence length99
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG3501920.18237601557495506No Hit
GTGCCAACCCCCCTGGCCCCCCCAAAAGGGGGGTGGTGCTGGTGCAATTG3315800.17268309739897997No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCA2611690.13601384843655892No Hit
GCTAAATAAACAAGAAAGCAAAATCAACAACCGCAAAAGTCTAAGACATG2046730.10659137340593952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG5426550.067.007292
AAGCAGT5567650.065.529691
CAGTGGT5568700.065.0698554
AGTGGTA5711850.063.3938755
GTGGTAT6018300.060.2111976
GCAGTGG6043600.060.099453
GGTATCA6167000.058.886938
TGGTATC6259900.057.9011767
GTATCAA7859800.046.5551079
CATGGGG3405700.033.5579824-25
AGAGTAC6063800.032.95536818-19
GCAGAGT6174350.032.5051716-17
TACATGG6111950.032.2210622-23
ACGCAGA6557350.031.01897214-15
CAACGCA6573550.031.0040312-13
ATCAACG6656350.030.6587510-11
CGCAGAG6217300.029.25440416-17
ACATGGG6104150.028.90394624-25
AGTACAT6897600.028.84076320-21
CAGAGTA6256700.028.72670618-19
Produced by FastQC (version 0.11.5)