FastQCFastQC Report
Tue 12 Jun 2018
HJJNMBCX2_l02_n01_Neset10x_adult2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJNMBCX2_l02_n01_Neset10x_adult2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192016477
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATG574500.018.15341
TTGAACG641350.018.149991
ACGAGGA539700.018.0711351
CATGGCG569200.017.9991381
GCGCGAT503050.017.972571
CTGCGGA409550.017.9368251
CTCGAAA505100.017.8996261
AGAAACC594950.017.802078
AGCTACC477800.017.719468
GACTAAC461950.017.7141421
TGACGGC408200.017.6895981
CTAAGAC610100.017.6697921
TGGCCAG681350.017.6045551
TGAGCCG511800.017.5194241
GGGCATC556650.017.5114211
TGGACGC534750.017.4970781
TGACTAG548600.017.4885311
GAATGAA449050.017.4526081
ACGAGCC567900.017.4515251
GACTGCG498950.017.4289951
Produced by FastQC (version 0.11.5)