FastQCFastQC Report
Tue 12 Jun 2018
HJJNMBCX2_l01_n02_Neset10x_adult2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJNMBCX2_l01_n02_Neset10x_adult2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193394461
Sequences flagged as poor quality0
Sequence length99
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG3544350.18327050225083746No Hit
GTGCCAACCCCCCTGGCCCCCCCAAAAGGGGGGTGGTGCTGGTGCAATTG3343290.17287413417698658No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCA2633030.13614815989998805No Hit
GCTAAATAAACAAGAAAGCAAAATCAACAACCGCAAAAGTCTAAGACATG2074790.10728280372000934No Hit
GGTGCATATGGTGCCAACCCCCCTGGCCCCCCCAAAAGGGGGGTGGTGCT1942980.10046720004043962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG5479400.066.9503862
AAGCAGT5617450.065.476861
CAGTGGT5614800.065.0692444
AGTGGTA5750300.063.58255
GTGGTAT6052750.060.4326676
GCAGTGG6097050.060.0748023
GGTATCA6201000.059.0728848
TGGTATC6293750.058.073487
GTATCAA7907350.046.6601689
CATGGGG3430500.033.60777724-25
AGAGTAC6090400.033.0599318-19
GCAGAGT6187450.032.53405416-17
TACATGG6151750.032.29136722-23
CAACGCA6592250.031.05140312-13
ACGCAGA6587250.030.94913714-15
ATCAACG6690150.030.67426510-11
CGCAGAG6236600.029.29532416-17
ACATGGG6124200.029.03925724-25
AGTACAT6938450.028.91565920-21
CAGAGTA6275950.028.8481518-19
Produced by FastQC (version 0.11.5)