FastQCFastQC Report
Tue 12 Jun 2018
HJJNMBCX2_l01_n01_Neset10x_adult2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJNMBCX2_l01_n01_Neset10x_adult2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193394461
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAACG643950.018.2289711
ACGAGGA531050.018.1115191
GCGCGAT513650.018.084441
CATGGCG566700.018.0648021
ACTGATG586550.018.034161
CTCGAAA510950.017.9886551
CTGCGGA409700.017.8077961
CTAAGAC613700.017.7485221
TGGCCAG682800.017.712181
AGAAACC597750.017.7110168
GGGCATC565650.017.6968941
TGAGCCG518100.017.661271
GACTAAC465900.017.6385971
AGCTACC483700.017.6272478
TGACGGC411750.017.596581
GATGCTA540100.017.4868761
TGACTAG559650.017.4714831
GAATGAA457100.017.45381
ACGAGCC567500.017.450251
TGGACGC533900.017.4477521
Produced by FastQC (version 0.11.5)