FastQCFastQC Report
Sat 26 May 2018
HJ5Y2BCX2_l02_n02_Neset10x_adult2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5Y2BCX2_l02_n02_Neset10x_adult2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108821823
Sequences flagged as poor quality0
Sequence length99
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG2262050.20786731352589083No Hit
GTGCCAACCCCCCTGGCCCCCCCAAAAGGGGGGTGGTGCTGGTGCAATTG1883350.17306730838353995No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCA1875900.17238270305396372No Hit
GGTGCATATGGTGCCAACCCCCCTGGCCCCCCCAAAAGGGGGGTGGTGCT1105690.10160553917572214No Hit
GCTAAATAAACAAGAAAGCAAAATCAACAACCGCAAAAGTCTAAGACATG1095880.10070406558066941No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG3249900.067.955032
AAGCAGT3311700.066.8736041
CAGTGGT3380600.065.139874
AGTGGTA3457850.063.6612855
GCAGTGG3640550.060.6091733
GTGGTAT3638400.060.5034876
GGTATCA3716150.059.3687558
TGGTATC3784400.058.217577
GTATCAA4714450.047.09259
CATGGGG2107600.033.47786724-25
AGAGTAC3673650.033.24480418-19
GCAGAGT3734700.032.72688316-17
TACATGG3718700.032.47142422-23
CAACGCA3942350.031.30270412-13
ACGCAGA3964200.031.09945314-15
ATCAACG3996350.030.94208110-11
CGCAGAG3752250.029.44011516-17
AGTACAT4164200.029.23403220-21
ACATGGG3705500.029.15629824-25
CAGAGTA3765400.029.12735618-19
Produced by FastQC (version 0.11.5)