FastQCFastQC Report
Sat 26 May 2018
HJ5Y2BCX2_l02_n01_Neset10x_adult2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5Y2BCX2_l02_n01_Neset10x_adult2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108821823
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGG1472600.13532212192401888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGGA296950.018.181141
TTGAACG350050.018.1779821
GCGCGAT282950.018.0884041
ACTGATG324850.017.99221
CTCGAAA291250.017.8148371
CTGCGGA221200.017.7516121
GACTAAC261450.017.7417721
CTAAGAC335150.017.705961
CATGGCG321000.017.6019361
TGGCCAG393100.017.5997071
AGAAACC341750.017.480058
TGACTAG303600.017.4711691
GATGCTA300600.017.4636171
TGCCAAA307850.017.4588381
GGGCATC312000.017.4187321
ACGAGCC314150.017.416681
AGCTACC268550.017.375238
GACTGCG280600.017.2767431
ACACTGA357100.017.230141
CCTACCA317250.017.2100321
Produced by FastQC (version 0.11.5)