FastQCFastQC Report
Sat 26 May 2018
HJ5Y2BCX2_l01_n02_Neset10x_adult1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5Y2BCX2_l01_n02_Neset10x_adult1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160480430
Sequences flagged as poor quality0
Sequence length99
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCA7323240.45633227677667615No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG5769980.35954415127127964No Hit
GTGCCAACCCCCCTGGCCCCCCCAAAAGGGGGGTGGTGCTGGTGCAATTG3569370.22241777393044124No Hit
GGTGCATATGGTGCCAACCCCCCTGGCCCCCCCAAAAGGGGGGTGGTGCT2206310.13748156083579785No Hit
GCTAAATAAACAAGAAAGCAAAATCAACAACCGCAAAAGTCTAAGACATG1937630.12073933251549737No Hit
GGTGCCAACCCCCCTGGCCCCCCCAAAAGGGGGGTGGTGCTGGTGCAATT1885270.11747662939337836No Hit
GCCAACCCCCCTGGCCCCCCCAAAAGGGGGGTGGTGCTGGTGCAATTGCA1860440.1159294002390198No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCAGC1635600.10191896918521468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT7090250.075.994481
AGCAGTG7358700.073.077912
CAGTGGT8189250.065.527634
AGTGGTA8451450.063.47285
GCAGTGG8479900.063.3534773
GTGGTAT8957800.060.0421076
GGTATCA9126400.058.928538
TGGTATC9139150.058.7886737
GTATCAA11082300.048.6589939
CATGGGG5702350.032.9981824-25
AGAGTAC9092600.032.82298318-19
GCAGAGT9177600.032.58548416-17
TACATGG9112200.032.4360722-23
CAACGCA9471100.031.8371112-13
ACGCAGA9469550.031.76719514-15
ATCAACG9541900.031.60569610-11
AGTACAT9802500.030.35920520-21
CATGGGA3042350.029.15751324-25
CGCAGAG9246950.029.10314416-17
CAGAGTA9200000.029.08148218-19
Produced by FastQC (version 0.11.5)