FastQCFastQC Report
Sat 26 May 2018
HJ5Y2BCX2_l01_n01_Neset10x_adult1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ5Y2BCX2_l01_n01_Neset10x_adult1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160480430
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA503800.018.4705248
AGGCGAC273450.018.3739458
GGAAAGC412450.018.0267091
GATGAAA463000.017.9353941
ATAACGC459600.017.9194721
CTGCGGA391350.017.9004971
GACTGCG474000.017.802921
GCGCGAT374350.017.795551
TTGAACG432800.017.679231
GGGAATG416100.017.6716121
ACTGATG418600.017.6614591
CATGGCG511150.017.6540221
TGAGCCG348100.017.592011
GACCTGG460600.017.542941
ACTGAAC401900.017.4933511
TGGCCAG464850.017.4842661
ATGCGAT465800.017.480191
GGGCATC525950.017.4009781
GCATGCG411050.017.3697991
TGACAAC446400.017.3689381
Produced by FastQC (version 0.11.5)