FastQCFastQC Report
Sat 26 May 2018
HHYCTBCX2_l02_n02_10x_ant_optic_lobes.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHYCTBCX2_l02_n02_10x_ant_optic_lobes.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187808211
Sequences flagged as poor quality0
Sequence length99
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT8824500.079.503491
AGCAGTG9219400.075.7918242
GCAGTGG9577400.072.693033
CAGTGGT9683250.071.8518144
TGGTATC9893350.070.008817
AGTGGTA10195000.068.169975
GGTATCA10210850.068.003388
GTGGTAT10236400.067.845486
GTATCAA10564850.065.926899
CATGGGG5815400.038.41759524-25
AGAGTAC9954250.037.84175518-19
GCAGAGT10012750.037.60923416-17
TACATGG9838700.037.57573322-23
CAACGCA10154550.037.35322612-13
ATCAACG10341500.036.70904510-11
ACGCAGA10516900.036.0788314-15
ACATGGG9656150.035.0814724-25
CAGAGTA9933600.034.99738318-19
TCAACGC10054500.034.7416212-13
AGTACAT10957600.034.19667420-21
Produced by FastQC (version 0.11.5)