FastQCFastQC Report
Sat 26 May 2018
HHYCTBCX2_l02_n01_10x_ant_optic_lobes.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHYCTBCX2_l02_n01_10x_ant_optic_lobes.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187808211
Sequences flagged as poor quality0
Sequence length27
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCTCTCTCTCTCTCTCTCTCTCTC6263280.33349340620682444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAC571850.018.3755191
GGGAGAT564350.018.0428141
AAACCTG725600.018.007581
ATGGGAG534450.017.9955231
CATGGCG549300.017.9732231
TGACGGA351350.017.9571610
CTGCGGA452750.017.942871
GATGAAA550900.017.9305841
GCTGCAG683150.017.9204391
TGAGCCG433400.017.8590341
GGAAAGC456350.017.7366731
CGATTGA734100.017.678511
GGGAATG445650.017.6707571
ACTGATG459650.017.6597671
CAATGGA426850.017.57958
TGACGGC376100.017.5514771
ATGACGG360600.017.517599
CGCTGGA477300.017.4173281
GCCGAAG327950.017.403223
GACTGCG460550.017.3209781
Produced by FastQC (version 0.11.5)