FastQCFastQC Report
Sat 26 May 2018
HHYCTBCX2_l01_n02_10x_ant_optic_lobes.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHYCTBCX2_l01_n02_10x_ant_optic_lobes.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184050267
Sequences flagged as poor quality0
Sequence length99
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT8679700.079.488961
AGCAGTG9071150.075.832272
GCAGTGG9429100.072.801093
CAGTGGT9547900.071.875964
TGGTATC9780250.070.107817
AGTGGTA10068350.068.244525
GGTATCA10094150.068.167068
GTGGTAT10098150.068.046076
GTATCAA10421550.066.156799
CATGGGG5695450.038.42072324-25
AGAGTAC9784500.037.8531318-19
TACATGG9657200.037.6766522-23
GCAGAGT9864700.037.62148316-17
CAACGCA9994450.037.42930612-13
ATCAACG10205850.036.76567510-11
ACGCAGA10357850.036.0773414-15
ACATGGG9480150.035.1523724-25
CAGAGTA9769500.035.035218-19
TCAACGC9913400.034.7923712-13
AGTACAT10775550.034.2466920-21
Produced by FastQC (version 0.11.5)