FastQCFastQC Report
Sat 26 May 2018
HHYCTBCX2_l01_n01_10x_ant_optic_lobes.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHYCTBCX2_l01_n01_10x_ant_optic_lobes.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184050267
Sequences flagged as poor quality0
Sequence length27
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCTCTCTCTCTCTCTCTCTCTCTC6284970.3414811672074347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAC564200.018.190661
TGACGGA338100.018.1137610
GCTGCAG669300.018.091321
AAACCTG706000.018.0669041
GGGAGAT550750.018.0592541
CTGCGGA447500.018.0222871
CATGGCG543750.018.008931
ATGGGAG540950.017.9833721
GATGAAA544400.017.9293821
TGAGCCG419450.017.863971
ACTGATG455950.017.808411
GGAAAGC438350.017.720881
CGATTGA723400.017.6541791
TGACGGC371700.017.6368641
ATGACGG349850.017.538299
GGGAATG437950.017.5158061
CAATGGA415300.017.5113228
GAATGAA413500.017.395061
TGGACGC435850.017.385121
GGGCATC504750.017.3387071
Produced by FastQC (version 0.11.5)