Highlight Samples

Regex mode off

    Rename Samples

    Click here for bulk input.

    Paste two columns of a tab-delimited table here (eg. from Excel).

    First column should be the old name, second column the new name.

    Regex mode off

      Show / Hide Samples

      Regex mode off

        Export Plots

        px
        px
        X

        Download the raw data used to create the plots in this report below:

        Note that additional data was saved in multiqc_data when this report was generated.


        Choose Plots

        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        Save Settings

        You can save the toolbox settings for this report to the browser.


        Load Settings

        Choose a saved report profile from the dropdown box below:

        About MultiQC

        This report was generated using MultiQC, version 1.0.dev0

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2018-08-25, 01:08 based on data in: /beegfs/mk5636/logs/html/HHL33BGX7/merged


        Welcome! Not sure where to start?   Watch a tutorial video   (6:06)

        General Statistics

        Showing 29/29 rows and 3/5 columns.
        Sample Name% Dups% GCM Seqs
        HHL33BGX7_n01_10_Input_TGA1_5_R1
        51.8%
        46%
        18.5
        HHL33BGX7_n01_11_C_R_NLP7_nlp7_GR
        38.3%
        43%
        33.3
        HHL33BGX7_n01_12_C_R_NLP7_nlp7_H3K27Ac
        28.6%
        42%
        37.3
        HHL33BGX7_n01_13_C_R_TGA1_Col_GR
        60.7%
        45%
        33.1
        HHL33BGX7_n01_14_C_R_TGA1_Col_H3K27Ac
        36.0%
        42%
        36.2
        HHL33BGX7_n01_15_C_R_bZIP1_Col_GR
        63.1%
        46%
        16.2
        HHL33BGX7_n01_16_C_R_bZIP1_Col_H3K27Ac
        37.7%
        44%
        20.9
        HHL33BGX7_n01_17_C_R_EV_Col_GR
        78.7%
        47%
        16.6
        HHL33BGX7_n01_18_C_R_EV_Col_H3K27Ac
        34.4%
        42%
        17.8
        HHL33BGX7_n01_19_C_R_EV_nlp7_GR
        64.6%
        46%
        17.2
        HHL33BGX7_n01_1_ChIP_NLP7_5_R1
        40.6%
        42%
        13.8
        HHL33BGX7_n01_20_C_R_EV_nlp7_H3K27Ac
        37.9%
        43%
        12.7
        HHL33BGX7_n01_21_col_dam_GR_r1
        48.5%
        46%
        12.8
        HHL33BGX7_n01_22_col_dam_NLP7_r1
        41.0%
        48%
        14.9
        HHL33BGX7_n01_23_nlp7_dam_GR_r1
        37.6%
        43%
        14.5
        HHL33BGX7_n01_24_nlp7_dam_NLP7_r1
        52.7%
        52%
        12.7
        HHL33BGX7_n01_25_col_dam_GR_r2
        47.7%
        45%
        9.6
        HHL33BGX7_n01_26_col_dam_NLP7_r2
        40.8%
        48%
        13.9
        HHL33BGX7_n01_27_nlp7_dam_GR_r2
        38.9%
        44%
        9.6
        HHL33BGX7_n01_28_nlp7_dam_NLP7_r2
        57.6%
        52%
        9.9
        HHL33BGX7_n01_2_ChIP_NLP7_10_R1
        40.7%
        42%
        12.6
        HHL33BGX7_n01_3_ChIP_NLP7_30_R1
        40.1%
        42%
        12.0
        HHL33BGX7_n01_4_ChIP_NLP7_180_R1
        40.9%
        42%
        11.6
        HHL33BGX7_n01_5_ChIP_TGA1_5_R1
        33.8%
        41%
        10.1
        HHL33BGX7_n01_6_ChIP_TGA1_10_R1
        39.7%
        42%
        12.0
        HHL33BGX7_n01_7_ChIP_TGA1_30_R1
        39.0%
        41%
        13.3
        HHL33BGX7_n01_8_ChIP_TGA1_180_R1
        48.6%
        42%
        14.2
        HHL33BGX7_n01_9_Input_NLP5_5_R1
        50.1%
        47%
        22.6
        HHL33BGX7_n01_undetermined
        70.1%
        44%
        16.2

        Demultiplexing Report

        Demultiplexing Report
        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool.

        Showing 29/29 rows and 2/2 columns.
        Sample NameTotal Read CountPortion (%)
        undetermined_library
        16,176,280
        3.3
        1_ChIP_NLP7_5_R1
        13,750,532
        2.8
        2_ChIP_NLP7_10_R1
        12,573,715
        2.5
        3_ChIP_NLP7_30_R1
        11,963,799
        2.4
        4_ChIP_NLP7_180_R1
        11,635,140
        2.3
        5_ChIP_TGA1_5_R1
        10,060,488
        2.0
        6_ChIP_TGA1_10_R1
        11,956,531
        2.4
        7_ChIP_TGA1_30_R1
        13,255,406
        2.7
        8_ChIP_TGA1_180_R1
        14,240,430
        2.9
        9_Input_NLP5_5_R1
        22,583,170
        4.6
        10_Input_TGA1_5_R1
        18,485,975
        3.7
        11_C_R_NLP7_nlp7_GR
        33,304,218
        6.7
        12_C_R_NLP7_nlp7_H3K27Ac
        37,258,005
        7.5
        13_C_R_TGA1_Col_GR
        33,128,234
        6.7
        14_C_R_TGA1_Col_H3K27Ac
        36,154,853
        7.3
        15_C_R_bZIP1_Col_GR
        16,187,146
        3.3
        16_C_R_bZIP1_Col_H3K27Ac
        20,896,892
        4.2
        17_C_R_EV_Col_GR
        16,620,951
        3.4
        18_C_R_EV_Col_H3K27Ac
        17,765,305
        3.6
        19_C_R_EV_nlp7_GR
        17,215,656
        3.5
        20_C_R_EV_nlp7_H3K27Ac
        12,676,876
        2.6
        21_col_dam_GR_r1
        12,823,522
        2.6
        22_col_dam_NLP7_r1
        14,858,667
        3.0
        23_nlp7_dam_GR_r1
        14,488,023
        2.9
        24_nlp7_dam_NLP7_r1
        12,731,386
        2.6
        25_col_dam_GR_r2
        9,614,508
        1.9
        26_col_dam_NLP7_r2
        13,902,025
        2.8
        27_nlp7_dam_GR_r2
        9,635,793
        1.9
        28_nlp7_dam_NLP7_r2
        9,895,865
        2.0

        Run Statistics

        Run Statistics

        Showing 1/1 rows and 4/4 columns.
        Sample NameTotal # of Single-End ReadsTotal # PF Reads% Undetermined % PhiX Aligned
        4.0
        551548480.0
        495,839,391
        3.3
        2.0

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Quality Histograms
        29
        0
        0

        The mean quality value across each base position in the read. See the FastQC help.

        Created with Highcharts 5.0.6Position (bp)Phred ScoreChart context menuExport PlotMean Quality Scores102030405060700510152025303540Created with MultiQC

        Per Sequence Quality Scores
        29
        0
        0

        The number of reads with average quality scores. Shows if a subset of reads has poor quality. See the FastQC help.

        Created with Highcharts 5.0.6Mean Sequence Quality (Phred Score)CountChart context menuExport PlotPer Sequence Quality Scores0510152025303505M10M15M20M25MCreated with MultiQC

        Per Base Sequence Content
        17
        4
        8

        The proportion of each base position for which each of the four normal DNA bases has been called. See the FastQC help.

        Click a heatmap row to see a line plot for that dataset.

        rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content
        14
        6
        9

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content. See the FastQC help.

        Created with Highcharts 5.0.6%GCCountChart context menuExport PlotPer Sequence GC Content0102030405060708090100012345678Created with MultiQC

        Per Base N Content
        29
        0
        0

        The percentage of base calls at each position for which an N was called. See the FastQC help.

        Created with Highcharts 5.0.6Position in Read (bp)Percentage N-CountChart context menuExport PlotPer Base N Content0102030405060700123456Created with MultiQC

        Sequence Length Distribution
        29
        0
        0

        All samples have sequences of a single length (76bp).


        Sequence Duplication Levels
        1
        19
        9

        The relative level of duplication found for every sequence. See the FastQC help.

        Created with Highcharts 5.0.6Sequence Duplication Level% of LibraryChart context menuExport PlotSequence Duplication Levels123456789>10>50>100>500>1k>5k>10k+020406080100Created with MultiQC

        Overrepresented sequences
        13
        16
        0

        The total amount of overrepresented sequences found in each library. See the FastQC help for further information.

        Created with Highcharts 5.0.6Percentage of Total SequencesChart context menuExport PlotOverrepresented sequencesTop over-represented sequenceSum of remaining over-represented sequencesHHL33BGX7_n01_10_Input_TGA1_5_R1HHL33BGX7_n01_12_C_R_NLP7_nlp7_H3K27AcHHL33BGX7_n01_14_C_R_TGA1_Col_H3K27AcHHL33BGX7_n01_16_C_R_bZIP1_Col_H3K27AcHHL33BGX7_n01_18_C_R_EV_Col_H3K27AcHHL33BGX7_n01_1_ChIP_NLP7_5_R1HHL33BGX7_n01_21_col_dam_GR_r1HHL33BGX7_n01_23_nlp7_dam_GR_r1HHL33BGX7_n01_25_col_dam_GR_r2HHL33BGX7_n01_27_nlp7_dam_GR_r2HHL33BGX7_n01_2_ChIP_NLP7_10_R1HHL33BGX7_n01_4_ChIP_NLP7_180_R1HHL33BGX7_n01_6_ChIP_TGA1_10_R1HHL33BGX7_n01_8_ChIP_TGA1_180_R1HHL33BGX7_n01_undetermined0%10%20%2.5%5%7.5%12.5%15%17.5%22.5%Created with MultiQC

        Adapter Content
        15
        13
        1

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. See the FastQC help. Only samples with ≥ 0.1% adapter contamination are shown.

        Created with Highcharts 5.0.6Position% of SequencesChart context menuExport PlotAdapter Content5101520253035404550556002.557.51012.515Created with MultiQC