FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16176280
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG475880.2941838296567567No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG386750.068.686071
GTCGCGG391900.068.086082
CGGCCGA392950.068.023846
TCGCGGC394200.067.580793
GGCCGAG400200.066.2841957
CGCGGCC406600.066.204924
GCGGCCG406800.065.8455055
GAGGATC403100.065.3559411
CGAGGAT405350.065.0794810
CCGAGGA422300.062.4176569
AGGATCA139100.058.98971612
GCCGAGG468300.056.9440548
AGGATCC117900.056.92399212
AGGATCG67450.056.80580512
AGGATCT154000.051.60086412
GGATCGT21200.046.54508613
GGATCCA55400.044.71817413
GGATCAC33550.043.90865313
GGATCAG38400.043.37468313
GGATCGC20250.042.8535913
Produced by FastQC (version 0.11.5)