FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_9_Input_NLP5_5_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_9_Input_NLP5_5_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22583170
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT338100.14971325991878023TruSeq Adapter, Index 18 (97% over 40bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC74500.036.88507546
TATGCCG76300.035.6958248
GTATGCC77650.035.4354247
TCCGCAC92850.032.0798335
GCCGTCT77800.031.67782451
CCGCACA92850.031.43926436
CGTCCGC96450.030.9544833
GTCCGCA97750.030.50720634
CGCACAT97200.029.31472437
CTCGTAT92850.029.14233244
TCGTATG96150.028.76122745
ACGTCCG110150.027.13626532
ATGCCGT104700.026.01317249
TCTCGTA101850.025.95013243
ATCTCGT114650.023.26596542
CATCTCG118150.022.78409641
GCACATC123350.022.67467338
TGGGGGG34300.022.449031
TCACGTC136050.022.20110130
CACATCT122750.022.01587539
Produced by FastQC (version 0.11.5)