FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_8_ChIP_TGA1_180_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_8_ChIP_TGA1_180_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14240430
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGC1103750.7750819322169344TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATG256700.1802614106456055TruSeq Adapter, Index 3 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG180150.051.4535246
CGTATGC181150.051.34267844
ATGCCGT180400.050.80098747
GTATGCC187400.049.81748645
GCCGTCT170950.049.35084549
TGCCGTC180600.049.27231248
CTCGTAT179250.048.3354542
CGTAGAG211400.048.26303533
GTCACGT211800.048.11994629
ACGTAGA214800.047.6452532
TCGTATG197050.047.38113443
TCACGTA216900.047.1668230
CACGTAG217300.047.04794731
AGTCACG217200.047.03870428
TCTCGTA188050.045.68229341
CCGTCTT195600.043.38208850
ATCTCGT200550.042.5386940
GATCTCG203800.041.89394439
CTCCAGT253850.041.32158324
CAGTCAC248150.041.3130927
Produced by FastQC (version 0.11.5)