FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_7_ChIP_TGA1_30_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_7_ChIP_TGA1_30_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13255406
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC238310.1797832522066846TruSeq Adapter, Index 16 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTCC124150.051.11207233
TATGCCG60250.048.04759646
CCGTCCA130600.047.4089534
CGTATGC60100.046.76966544
TCACCCG138000.046.2861730
ATGCCGT58600.045.87855547
CACCCGT139400.045.82166331
CGTCCAT126850.044.09338435
AGTCACC147500.043.73528328
GTCACCC146600.043.57055729
GTATGCC67850.043.38780245
TGCCGTC59200.042.63548
ACCCGTC151650.042.05151432
ACTCCAG161100.041.1492323
CAGTCAC157800.040.9469627
CTCCAGT162150.040.6666124
GCCGTCT53400.040.31684549
TCCAGTC167500.039.40994625
CCAGTCA163900.039.1664426
ACGTCTG175400.038.1873215
Produced by FastQC (version 0.11.5)