FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_6_ChIP_TGA1_10_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_6_ChIP_TGA1_10_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11956531
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT937200.7838393928807611TruSeq Adapter, Index 13 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA231190.19335875932576096TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC144350.050.70848546
TATGCCG143300.050.5935848
GTATGCC148550.049.32356647
GCCGTCT136100.049.1288751
ATGCCGT145950.048.88342349
TGCCGTC144700.047.87810550
CTCGTAT149450.047.87695344
TCGTATG158650.046.53540445
TCTCGTA156450.045.76101343
ACAGTCA179500.044.75126332
CACAGTC181450.044.25048431
CCGTCTT155450.043.3732252
GTCACAG185050.043.2759629
CAGTCAA186950.043.00553533
ATCTCGT168650.042.9472842
TCACAGT188450.042.6251930
CAGTCAC189300.042.45512427
AGTCAAC191200.041.9397734
CTCCAGT196850.041.41134324
ACTCCAG197350.041.37771223
Produced by FastQC (version 0.11.5)