FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_5_ChIP_TGA1_5_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_5_ChIP_TGA1_5_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10060488
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC638270.6344324450265236TruSeq Adapter, Index 11 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG102390.10177438708738583TruSeq Adapter, Index 11 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG94500.055.71281446
CGTATGC97600.054.6241744
ATGCCGT95600.053.64451247
GTATGCC100050.053.42729645
CACGGCT110450.053.14219331
GTCACGG110600.052.84702729
TGCCGTC94200.052.54731848
AGTCACG112100.052.05164728
GCCGTCT91150.052.0394249
ACGGCTA117600.050.179332
CTCGTAT103500.049.95552442
CGGCTAC118550.049.65933233
ACGTCTG121800.049.3641115
GCACACG122350.049.2798411
TCGTATG110450.048.4618843
ACACGTC125500.048.1265813
CGTCTGA125950.047.6820116
ACTCCAG124750.047.66802623
CACACGT127000.047.530612
GCTACAT119050.047.0411435
Produced by FastQC (version 0.11.5)