FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_3_ChIP_NLP7_30_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_3_ChIP_NLP7_30_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11963799
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC591490.4943998139721338TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG91000.052.7392646
CGTATGC94250.051.17915744
GTATGCC95300.050.6161245
GCCGTCT92850.050.2214649
ATGCCGT96700.049.4522247
AGTCACG100050.048.87352828
TGCCGTC103300.045.92062448
CTCGTAT99550.045.8523542
CGATCAG107200.045.67735733
GTCACGA107200.045.57750329
TCGTATG110550.043.793443
TCTCGTA105750.042.66845341
CAGTCAC117450.041.8121727
CCGTCTT113750.041.27094750
CCAGTCA119150.041.12747226
CACGATC120850.040.45899631
TCACGAT121900.040.31164630
ACTCCAG123350.040.09482623
GATCTCG111000.040.0511639
CTCCAGT123850.040.04624
Produced by FastQC (version 0.11.5)