FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_2_ChIP_NLP7_10_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_2_ChIP_NLP7_10_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12573715
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC621330.49414989921435315TruSeq Adapter, Index 6 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG129340.10286538226769097TruSeq Adapter, Index 6 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG91700.050.16105746
CGTATGC93450.049.51961544
GCCGTCT84700.048.4820749
CACGCCA110300.048.10555631
ATGCCGT94500.047.6769147
GTATGCC97500.047.49854745
AGTCACG112500.047.293628
CTCGTAT101450.045.92648342
GTCACGC117200.045.51140629
TCACGCC117200.045.4221830
ACGCCAA118900.044.74580432
CGCCAAT120950.044.01598433
TGCCGTC99550.043.95755848
GCCAATA123650.042.99807734
TCTCGTA111150.042.7688441
TCGTATG109400.042.68639843
CAGTCAC130100.040.8689527
TATCTCG125600.039.8552339
CCAGTCA133200.039.81252326
TCCAGTC135550.039.5864925
Produced by FastQC (version 0.11.5)