FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_28_nlp7_dam_NLP7_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_28_nlp7_dam_NLP7_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9895865
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCGA3307000.069.303716
GCGGCCG3311050.069.202035
GAGGATC3274400.069.1793811
GGTCGCG3310850.069.059871
AGGATCT814750.069.056412
GGCCGAG3320950.069.009437
AGGATCA1040700.068.9921912
CGCGGCC3323150.068.920574
CGAGGAT3290050.068.9173210
AGGATCG651650.068.87253612
CCGAGGA3292650.068.85449
GCCGAGG3337450.068.685038
GTCGCGG3334600.068.62552
TCGCGGC3347150.068.354263
GGATCTA77200.067.7177613
GGATCGT183300.067.71236413
GGATCCG221050.067.59376513
AGGATCC796600.067.5373512
GGATCTT239600.067.51594513
GGATCAT288750.067.3664913
Produced by FastQC (version 0.11.5)