FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_27_nlp7_dam_GR_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_27_nlp7_dam_GR_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9635793
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGCGGCCGAGGATCCGCCTAGGCTGTCCCGAGTGTGAGCGAGGTGTG405530.4208579408046645No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCT379270.3936053835942719No Hit
GGTCGCGGCCGAGGATCGGGTTGCGGTTTAAGTTCTTATACTCAATCATA172380.17889549931178472No Hit
GGTCGCGGCCGAGGATCTTAAAGGCGTAAGAATTGTATCCTTGTTAGAAG170730.17718313375972272No Hit
GGTCGCGGCCGAGGATCTTAAAAGCCTAAGTAGTGTTTCCTTGTTAGAAG170350.17678877078409636No Hit
GGTCGCGGCCGAGGATCGGGTTGCGGTTTAAGTTGTTATACTCAATCATA164730.1709563499340428No Hit
GGTCGCGGCCGAGGATCAAGTCATATTCGACTCCAAAACACTAACCAACC110350.11452093252729692No Hit
GGTCGCGGCCGAGGATCCGGTTGCGGTTTAAGTTCTTATACTCAATCATA98930.10266928731241944No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG2918600.069.77431
CGCGGCC2941550.069.307514
GTCGCGG2939450.069.291542
GCGGCCG2941900.069.236225
TCGCGGC2941850.069.2040943
AGGATCT1001100.069.1703212
CGGCCGA2948900.069.147826
AGGATCA905150.069.0337312
GAGGATC2931650.069.0068211
AGGATCG299650.068.9804512
GGCCGAG2949650.068.9594047
GGATCTA158500.068.9471113
CGAGGAT2944300.068.7828410
CCGAGGA2943750.068.7647869
GCCGAGG2960250.068.7585758
GGATCTC237500.068.7471213
GGATCCG166350.068.7436513
GGATCAA343300.068.6902413
GGATCTT453500.068.6035713
GGATCCA303200.068.5421213
Produced by FastQC (version 0.11.5)