FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_26_col_dam_NLP7_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_26_col_dam_NLP7_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13902025
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGCGGCCGAGGATCCGCCTAGGCTGTCCCGAGTGTGAGCGAGGTGTG356000.2560778016152323No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCT350840.25236611213114635No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG3457450.069.327761
GCGGCCG3462600.069.093735
CGGCCGA3473800.069.018026
CGCGGCC3482850.068.859794
GGCCGAG3469350.068.713967
GTCGCGG3493650.068.629382
AGGATCT951650.068.6259812
GAGGATC3414800.068.5676611
AGGATCA1113350.068.5370312
TCGCGGC3500200.068.478473
GCCGAGG3484950.068.36328
CCGAGGA3428200.068.3568049
CGAGGAT3431350.068.3409610
AGGATCG492950.067.8958412
GGATCGT145850.067.0080813
GGATCTA130450.066.9787613
GGATCTT311150.066.9691313
GGATCTC269400.066.81377413
GGATCAT326450.066.7995313
GGATCAA358350.066.5849313
Produced by FastQC (version 0.11.5)