FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_25_col_dam_GR_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_25_col_dam_GR_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9614508
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCT863990.8986315264390023No Hit
GGTCGCGGCCGAGGATCCGCCTAGGCTGTCCCGAGTGTGAGCGAGGTGTG833950.867387077945122No Hit
GGTCGCGGCCGAGGATCGGGTTGCGGTTTAAGTTCTTATACTCAATCATA305480.3177281666414964No Hit
GGTCGCGGCCGAGGATCTTAAAAGCCTAAGTAGTGTTTCCTTGTTAGAAG283710.29508530233684344No Hit
GGTCGCGGCCGAGGATCGGGTTGCGGTTTAAGTTGTTATACTCAATCATA258870.2692493469244604No Hit
GGTCGCGGCCGAGGATCAAGTCATATTCGACTCCAAAACACTAACCAACC216620.22530534063729524No Hit
GGTCGCGGCCGAGGATCTTAAAGGCGTAAGAATTGTATCCTTGTTAGAAG208030.21637092610459113No Hit
GGTCGCGGCCGAGGATCTTAAAGGCGTAAGAATTGTATCCTTGTTAAAAG142720.14844233319063233No Hit
GGTCGCGGCCGAGGATCCATTTTGCCGACTTCCCTTGCCTACATTGTTCC141670.14735023362609923No Hit
GGTCGCGGCCGAGGATCTTATAAGCCTAAGTAGTGTTTCCTTGTTAGAAC131510.13678287022071228No Hit
GGTCGCGGCCGAGGATCTTATAAGCCTAAGTAGTGTTTCCTTGTTAGAAG97670.10158606139804555No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG2888600.069.8042451
CGCGGCC2916400.069.165384
GTCGCGG2915400.069.143862
TCGCGGC2914700.069.13733
GCGGCCG2913100.069.10085
CGGCCGA2923500.068.975866
GGCCGAG2919900.068.742987
GCCGAGG2924950.068.581228
CCGAGGA2901400.068.519199
CGAGGAT2903750.068.4649410
GAGGATC2906300.068.3699611
AGGATCT971750.068.2728912
AGGATCA813650.068.1383612
AGGATCG370250.067.620612
GGATCGG162600.066.94897513
GGATCGT84900.066.9333813
GGATCTA140550.066.5476313
GGATCTT495700.066.50320413
GGATCAA316600.066.2918313
GGATCAT241150.066.0074813
Produced by FastQC (version 0.11.5)