FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_24_nlp7_dam_NLP7_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_24_nlp7_dam_NLP7_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12731386
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCGA4063900.069.264126
GCGGCCG4061450.069.218895
GGTCGCG4090200.068.967691
GGCCGAG4044050.068.962937
GAGGATC3998950.068.9610211
CGCGGCC4099950.068.842874
AGGATCT1018850.068.8385712
CCGAGGA4013900.068.7887349
CGAGGAT4017550.068.7210110
AGGATCA1284100.068.7158212
AGGATCG792850.068.6616412
GCCGAGG4062250.068.5841758
GTCGCGG4111700.068.540612
TCGCGGC4125850.068.2784043
GGATCGT222400.067.4839713
GGATCCG262850.067.3763913
GGATCTT308400.067.25117513
GGATCAT359700.067.1644813
GGATCTC296950.067.0396913
GGATCAA372000.066.881513
Produced by FastQC (version 0.11.5)