FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_23_nlp7_dam_GR_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_23_nlp7_dam_GR_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14488023
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCT462030.31890479467074284No Hit
GGTCGCGGCCGAGGATCCGCCTAGGCTGTCCCGAGTGTGAGCGAGGTGTG440740.30420989806545723No Hit
GGTCGCGGCCGAGGATCGGGTTGCGGTTTAAGTTCTTATACTCAATCATA278900.19250383575454014No Hit
GGTCGCGGCCGAGGATCTTAAAAGCCTAAGTAGTGTTTCCTTGTTAGAAG264340.18245415540822926No Hit
GGTCGCGGCCGAGGATCTTAAAGGCGTAAGAATTGTATCCTTGTTAGAAG257000.1773879017171632No Hit
GGTCGCGGCCGAGGATCGGGTTGCGGTTTAAGTTGTTATACTCAATCATA251830.17381943692386465No Hit
GGTCGCGGCCGAGGATCAAGTCATATTCGACTCCAAAACACTAACCAACC187150.12917566461621438No Hit
GGTCGCGGCCGAGGATCCGGTTGCGGTTTAAGTTCTTATACTCAATCATA165320.11410804634973315No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG4306850.069.72291
CGCGGCC4319000.069.331274
GTCGCGG4325400.069.287172
TCGCGGC4322200.069.273463
GCGGCCG4299750.069.259975
CGGCCGA4316500.069.1517266
GGCCGAG4275950.068.9741747
AGGATCT1501700.068.96760612
GCCGAGG4282300.068.844128
AGGATCA1297900.068.8400612
CCGAGGA4256700.068.82419
GAGGATC4258500.068.7862111
CGAGGAT4265150.068.72793610
AGGATCG442500.068.6178312
GGATCTC337250.068.6162813
GGATCTA239150.068.5812213
GGATCTT708100.068.5577313
GGATCTG226900.068.45911413
GGATCCG245450.068.4034313
GGATCAA505350.068.3932813
Produced by FastQC (version 0.11.5)