FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_22_col_dam_NLP7_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_22_col_dam_NLP7_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14858667
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCT362590.24402592776323742No Hit
GGTCGCGGCCGAGGATCCGCCTAGGCTGTCCCGAGTGTGAGCGAGGTGTG338220.2276247256904001No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG4018250.069.321591
GCGGCCG3987400.069.129765
CGGCCGA4001150.069.116086
CGCGGCC4039600.068.906074
GGCCGAG3953100.068.785077
GTCGCGG4049450.068.6917952
GAGGATC3910200.068.64326511
GCCGAGG3964450.068.5025258
CCGAGGA3924400.068.486519
TCGCGGC4061800.068.475183
AGGATCT1106650.068.4680912
AGGATCA1280450.068.4117412
CGAGGAT3928700.068.4097710
AGGATCG564700.068.1516412
GGATCGT173600.067.2041813
GGATCTA144350.067.07686613
GGATCAT379600.066.8516313
GGATCTT372650.066.6053713
GGATCCA375300.066.53599513
GGATCTC319100.066.4876813
Produced by FastQC (version 0.11.5)