FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_21_col_dam_GR_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_21_col_dam_GR_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12823522
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCT1061620.8278692858327065No Hit
GGTCGCGGCCGAGGATCCGCCTAGGCTGTCCCGAGTGTGAGCGAGGTGTG1044320.814378452347179No Hit
GGTCGCGGCCGAGGATCCATTTTGCCGACTTCCCTTGCCTACATTGTTCC389720.30391026739767746No Hit
GGTCGCGGCCGAGGATCGGGTTGCGGTTTAAGTTCTTATACTCAATCATA362260.2824964935530192No Hit
GGTCGCGGCCGAGGATCTTAAAAGCCTAAGTAGTGTTTCCTTGTTAGAAG360350.2810070431508598No Hit
GGTCGCGGCCGAGGATCGGGTTGCGGTTTAAGTTGTTATACTCAATCATA310870.24242169974832187No Hit
GGTCGCGGCCGAGGATCAAGTCATATTCGACTCCAAAACACTAACCAACC276750.21581434492021773No Hit
GGTCGCGGCCGAGGATCTTAAAGGCGTAAGAATTGTATCCTTGTTAGAAG252600.19698176522799277No Hit
GGTCGCGGCCGAGGATCCGGTTAAAATTCCGGAACCGGGACGTGGCGGTT213830.16674826151505023No Hit
GGTCGCGGCCGAGGATCTTAAAGGCGTAAGAATTGTATCCTTGTTAAAAG176010.137255583918365No Hit
GGTCGCGGCCGAGGATCTTATAAGCCTAAGTAGTGTTTCCTTGTTAGAAC168580.1314615438722685No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG3903250.069.7905651
CGCGGCC3944650.069.078324
GTCGCGG3942000.069.059062
TCGCGGC3943400.069.036323
GCGGCCG3925400.069.031975
CGGCCGA3944800.068.855696
GGCCGAG3919100.068.606317
CCGAGGA3915900.068.393399
GCCGAGG3928000.068.376928
CGAGGAT3922400.068.2657810
GAGGATC3923050.068.19559511
AGGATCT1295050.068.019612
AGGATCA1088250.067.93198412
AGGATCG500750.066.93288412
GGATCGG202450.066.7549613
GGATCTA180150.066.238313
GGATCTT661750.066.1640113
GGATCCG335050.066.11243413
GGATCAA418000.065.9772313
GGATCTC290500.065.7952613
Produced by FastQC (version 0.11.5)