FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_20_C_R_EV_nlp7_H3K27Ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_20_C_R_EV_nlp7_H3K27Ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12676876
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCGA20750.038.1133736
GGTCGCG20650.037.457141
GTCGCGG22400.035.3060532
CGTATGC19350.027.49719644
TATGCCG19350.027.31672546
GTATGCC21150.025.81913845
ATGCCGT21950.024.7197247
GCCGTCT23500.021.7488649
TGCCGTC24500.021.14700548
TCGCGGC44900.017.9253863
GGCCGAG52400.016.227767
CCGTCTT36700.014.21254150
TCTCGTA36150.013.16968141
CGAGGAT64700.012.76413410
CGCGGCC71700.012.3965714
TCGTATG46000.012.25269243
GAGGATC67250.012.12403711
ATCTCGT44050.011.99994140
GCCGAGG75200.011.4007088
CTCGTAT46150.011.30219142
Produced by FastQC (version 0.11.5)