FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_1_ChIP_NLP7_5_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_1_ChIP_NLP7_5_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13750532
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC177490.12907864219362566TruSeq Adapter, Index 5 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG56400.050.1516246
CGTATGC56900.047.9255244
CACAGTG129900.045.88756633
GTCACAC132600.045.1892929
GTATGCC64550.044.57814445
ACACAGT139500.042.8804132
ATGCCGT58400.041.84187347
CTCCAGT146800.041.05505424
CAGTGAT138550.040.77578435
CAGTCAC147500.040.6018627
GCCGTCT46850.040.27612749
ACGTCTG152100.039.8949415
CACACAG150400.039.84152631
ACTCCAG152050.039.75302523
CCAGTCA150650.039.683226
TCCAGTC154000.039.15960325
ACAGTGA150100.039.0828334
CGTCTGA156600.038.77087816
CACACGT160650.037.79126412
ACACGTC161850.037.46782713
Produced by FastQC (version 0.11.5)