FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_19_C_R_EV_nlp7_GR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_19_C_R_EV_nlp7_GR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17215656
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT28750.029.83331949
GTATGCC30800.028.98185745
CGTATGC31200.028.72249444
TGCCGTC30600.028.60187348
TATGCCG31400.028.20567346
ATGCCGT31100.028.141347
CCGTCTT42400.020.5591750
TCTCGTA47650.017.8525541
ATCTCGT51600.016.21451240
GCTTGAA66050.012.55864859
TGCTTGA66750.012.47999858
TCGTATG79250.012.05981143
AGATCTC70300.011.85147238
GTCGCGG14400.011.664042
CTCGTAT79950.011.20923342
GGTCGCG15200.010.8222271
AGTCACC87800.010.68459628
CGGCCGA16900.010.1456436
CTGCTTG81250.010.08071757
CCAAAAG103950.09.32682933
Produced by FastQC (version 0.11.5)