FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_17_C_R_EV_Col_GR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_17_C_R_EV_Col_GR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16620951
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGGG23900.021.23777439
GCATCGG27200.019.17592238
GTCACAT90250.013.4569129
GGGGTGT10700.013.41376652
TGAGCAT89600.013.08653635
GGGTGTA9600.012.395802559
CGGGGGT11850.011.81634442
GCATCGC19900.010.90630138
ATGGGAG114350.010.8351951
TGGCGGG18000.010.69670448
ACATGAG120650.010.64676132
TGGGAGA122700.010.0104242
TGCGGGG10550.09.9547449
GTGTGGG17450.09.82897355
GAGAGCT128500.09.6130565
CACATGA135100.09.40406631
AGAGCTC129800.09.355026
GTATGCC12550.09.20449445
CATGAGC145500.08.99670433
TAGTGTC75250.08.93160846
Produced by FastQC (version 0.11.5)