FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_15_C_R_bZIP1_Col_GR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_15_C_R_bZIP1_Col_GR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16187146
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC22350.011.74720944
GTATGCC23700.011.52118745
GCCGTCT22100.010.29681549
TATGCCG26400.010.21028246
TGCCGTC24700.010.2050448
ATGCCGT22600.010.06866747
TAGCATC52150.09.32963236
GGTAGCA67150.08.60078134
GTAGCAT63500.08.54388235
CACGGTA89550.08.51871770
ACGGTAG82150.08.22115770
GTCACGG152100.08.14436670
CGGTAGC73150.08.08454670
AGTCACG165500.07.65409470
CATCTCG48450.07.58682239
ATCTCGT45950.07.46636140
CCGTCTT36150.07.3600950
TCACGGT142700.07.06239170
TCTCGTA44150.06.89860141
GCATCTC53800.06.897355638
Produced by FastQC (version 0.11.5)