FastQCFastQC Report
Sat 25 Aug 2018
HHL33BGX7_n01_10_Input_TGA1_5_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHL33BGX7_n01_10_Input_TGA1_5_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18485975
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT190140.10285635461478229TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC47350.035.85613646
GTATGCC51100.033.02037447
TATGCCG53600.031.48033148
ATGCCGT54200.031.26093549
TGCCGTC57650.029.20806550
TCTCGTA65550.024.35314843
TCGTATG72350.023.8530745
CTCGTAT68650.023.6087444
GCCGTCT72200.023.17630651
ATCTCGT73450.021.06609242
TGAAACG84050.020.9051735
CACGTGA85000.020.42318331
CGTGAAA88950.019.71400333
CCGTCTT86850.019.42811652
TCACGTG90050.019.27759430
GAAACGA89500.018.85016836
GTCACGT93150.018.56074329
TGCTTGA97650.016.59510660
GCTTGAA99350.016.38164561
AGTCACG117300.014.92005128
Produced by FastQC (version 0.11.5)