FastQCFastQC Report
Fri 29 Jun 2018
HH5HLBGX7_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5HLBGX7_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences591937034
Sequences flagged as poor quality0
Sequence length99
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7326139412.376551861426531No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37052600.6259550910274689No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG14769310.24950812589299826No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCA8199380.13851777349683447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT17199400.071.524531
AGCAGTG17249850.071.069172
CAGTGGT18527950.065.980084
GCAGTGG19103550.064.21063
AGTGGTA19868900.061.7440455
GTGGTAT20442950.059.935496
TGGTATC20403950.059.8877
GGTATCA20576200.059.4651878
GTATCAA25079750.048.6687749
CATGGGG12758850.033.69748324-25
AGAGTAC20317700.033.2017618-19
GCAGAGT20569300.032.8565816-17
TACATGG20361050.032.76791422-23
CAACGCA21031500.032.067512-13
ATCAACG21311100.031.7077610-11
ACGCAGA21555050.031.31012714-15
CAGAGTA20465250.029.94587118-19
ACATGGG20243750.029.82218724-25
CGCAGAG20948500.029.3775916-17
GAGTACA21129600.028.95878820-21
Produced by FastQC (version 0.11.5)