FastQCFastQC Report
Fri 29 Jun 2018
HH5HLBGX7_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5HLBGX7_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences591937034
Sequences flagged as poor quality0
Sequence length8
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGCCG7533477812.726822900558709No Hit
TTCCCGAC7354269912.424074652507718No Hit
NNNNNNNN7327729912.379238802618996No Hit
CCCACAGT6229423210.523793650660487No Hit
GCAGTAGA6025742010.179700971370547No Hit
AGTAGTCT589452209.958021987858931No Hit
CAGTACTG551759479.321252739864896No Hit
TATGATTC492615488.322092582570193No Hit
ATGCTGAA455547357.6958751325567505No Hit
GGGGGGGG189889523.2079344439192496No Hit
TTCCCGCC8901550.1503800149121942No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)