FastQCFastQC Report
Fri 29 Jun 2018
HH5HLBGX7_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5HLBGX7_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences591937034
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA1386900.017.48838
GGGAATG1330800.016.6952041
CTGCGGA1238300.016.6384871
GACTAAC1348200.016.4020731
ACTGAAC1451850.016.340461
TTGAACG1391450.016.2980161
TGACTAG1541900.016.2650831
ATGCGAT1479100.016.2643451
GATGAAA1443600.016.2508341
GAATGAA1371000.016.2327941
AGAAACC1552000.016.1526178
GGGACCT1150750.016.1433431
CTCGAAA1402750.016.110921
CGCTGGA1271100.016.1013681
ATAGACC1432800.016.0721531
GACCTGG1380450.016.0160541
CCTACCA1618600.015.9646661
AGGCGAC462000.015.9423668
TTGGCGC1131200.015.80761510
GGATTAC1514000.015.7931281
Produced by FastQC (version 0.11.5)