FastQCFastQC Report
Wed 18 Jul 2018
HH5CFBGX7_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18810751
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3057151.6252142192515335No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG400950.2131493846258451No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG393640.2092633090512973No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG371100.19728079968737025No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA292990.15575667340448024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG55350.053.094081
ACGTCTG38750.036.37340515
GCACACG41450.035.52042811
CACACGT42000.034.05571712
ACACGTC44900.031.70068413
CGTCTGA48950.028.57971416
CACGTCT50300.028.0888514
AGCACAC55850.027.05093810
AGCGGGG7250.025.6670631
TCGGAAG68600.024.3814093
AGAGCAC68200.022.818868
CTGAACT62200.022.71747419
CGGGGGG123350.022.173621
GATCGGA78300.021.47891
ATCGGAA79800.021.2734092
CGGAAGA84200.020.2779774
GAACTCC66650.019.62686321
TGGGGGG165250.017.4650021
CAGTCAC56250.016.29277827
TGAACTC86150.016.11770220
Produced by FastQC (version 0.11.5)