FastQCFastQC Report
Wed 18 Jul 2018
HH5CFBGX7_n01_NutriNet.AtRep.Mh-1_HN_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_NutriNet.AtRep.Mh-1_HN_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14871243
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTCGTATGC304830.20497950305835227TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG51700.056.32740846
CGTATGC57700.050.95611244
GTATGCC60950.048.12367245
ATGCCGT62800.046.25920547
CTAATCG64950.045.7484233
ACGTCTG68950.043.5513915
TCGTATG66700.043.45119543
TGCCGTC67300.043.4259848
GCACACG71900.042.1542111
GCCGTCT74050.039.4189149
CACACGT76750.039.07985312
ACTAATC79800.037.58619732
ACACGTC80900.036.9887413
CTCGTAT65350.036.7429742
CACTAAT83250.036.15481631
GTCACTA88650.033.75543229
AGTCACT89950.033.54006228
CACGTCT90500.033.3743414
CGTCTGA90600.033.1442416
AGCACAC95600.032.25298710
Produced by FastQC (version 0.11.5)