FastQCFastQC Report
Wed 18 Jul 2018
HH5CFBGX7_n01_NutriNet.AtRep.Mh-1_HN_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_NutriNet.AtRep.Mh-1_HN_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25353007
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG47250.046.81587646
CGTATGC56300.039.6020344
GTATGCC61100.036.6626245
ATGCCGT66950.033.0924847
TCGTATG69650.031.86089743
ACGTCTG79950.029.41754515
GTCACGT81450.028.40317229
GCACACG83600.028.04915811
TGCCGTC80200.027.6251548
TCACGTA84900.027.0840330
CACGTAG86900.026.09826931
CACACGT93050.025.8023912
AGTCACG92600.025.09652128
GCCGTCT91750.024.18502249
ACACGTC98000.024.17778213
CTCGTAT83000.023.82640542
CGTAGAG108150.021.26148633
AGCACAC115550.020.8689510
ACGTAGA111450.020.7261932
CGTCTGA117700.020.63658116
Produced by FastQC (version 0.11.5)