FastQCFastQC Report
Wed 18 Jul 2018
HH5CFBGX7_n01_NutriNet.AtRep.Mh-1_HN_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_NutriNet.AtRep.Mh-1_HN_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13525377
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG14100.026.80956646
ACGTCTG36300.020.1513415
GCACACG37150.019.97305511
GTATGCC20550.018.90599445
CGTATGC20000.018.72601344
CACACGT39800.018.64305312
ATGCCGT24600.015.93529247
TGCCGTC27350.014.71694448
ACACGTC51200.014.69713813
CACGTCT53000.014.06591614
CGTCTGA54600.013.91013616
GCGGGGA8900.013.7659621
TCGTATG28350.013.70459843
GGGGCGC6550.013.35856429
AGCACAC60500.013.01612810
CGCGTAT11900.012.3546611
GCCGAAT19150.012.0643431
GAGCACA66200.012.05400859
CTCGTAT32700.011.88155442
GGCGAAT29600.011.4711921
Produced by FastQC (version 0.11.5)