FastQCFastQC Report
Wed 18 Jul 2018
HH5CFBGX7_n01_NutriNet.AtRep.Kn-0_HN_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_NutriNet.AtRep.Kn-0_HN_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15323401
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGC350540.22876122604896915TruSeq Adapter, Index 23 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG62350.057.7078746
GTATGCC69000.052.4512145
CGTATGC69450.051.96049544
ATGCCGT70500.051.135847
TCGTATG78100.046.29537243
GCACACG82850.046.2148911
ACGTCTG83400.045.90981715
TGCCGTC79750.044.89747648
GCCGTCT82650.042.98221649
CACACGT89500.042.85925712
ACACGTC92500.041.2800413
CTCGTAT73700.039.98790742
CCAACTA97600.038.1902633
CACGTCT100350.038.12061314
CGTCTGA104250.036.89571416
AGCACAC106100.036.6152410
AACTAAT98700.036.06374735
TCGGAAG108300.035.616863
ACTAATC81750.035.10729636
GAGCACA114200.034.416719
Produced by FastQC (version 0.11.5)